Calibrating microscope camera without a calibration slide

[Knebo]

Hi all,

As I am planning my first intervention on pallet jewels, I was looking into measuring precise distances with my Amscope microscope and Hayear camera. Since I hadn't bought the dedicated calibration slide for this purpose, I figured that it should be possible without.

Here's a quick walk-through.

0. Switch on camera and put an object of which you know the exact size (to the 0.01mm at least). Ideally something as large as possible without exceeding the field of view (in the computer). I use a Seitz pusher that is supposed to be 1.95mm and use maximum magnification on the microscope.

1. open Hayear software

2. select 100% here:

 

3. Go to "Options" and "Calibrate"

 

 

4. Enter the correct unit and amount of the actual object. And draw the red line on the screen to match the respective distance. "OK" and done.
NOTE: the "Magnification" field has no real importance since the camera software doesn't know what we are doing with the magnification handle on the scope. So the calibration is only useful at a given magnification level. Once changing the magnification on the scope, you will NOT be able to measure distances anymore.

 

 

Now a test:

This pusher is supposed to be 0.75mm.

I select this symbol:

 

Yep, that's pretty good